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Regulator Of Chromosome Condensation 1

Methods of identifying compounds for their ability to function as protein condensation inhibitors, as well as the use of such protein condensation inhibitors to treat phase transition disease states, are also disclosed. In one embodiment, a method of identifying compounds comprises assaying the compound in an aqueous protein solution to determine whether the sign of 'delta'E is positive.


In this method, the assaying may include the steps of measuring a change in phase separation temperature and determining whether protein condensation occurs upon raising or lowering the temperature of the aqueous protein solution. Preferably, the aqueous protein solution comprises a protein associated with a phase transition disease state, such as sickle hemoglobin.

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In another embodiment, a method of identifying compounds involves assaying the compound in at least two distinct aqueous protein solutions for its ability to prevent or delay a temperature dependent phase transition to a condensed state in each of the distinct aqueous protein solutions.

Examples of suitable proteins for the distinct aqueous protein solutions include lysozyme and sickle hemoglobin. Priority number:. The reaction appears to be a transamidation reaction in which incorporation of the fatty acid moiety results in partial cleavage of the polypeptide chain and in formation of methyl alcohol.

Taming Protein Condensation | Department of Chemistry

Other solvents which can dissolve both fatty acid esters and collagen-derived proteins, such as ethylene glycol, can be used to advantage in this reaction. Unable to display preview. Download preview PDF. Skip to main content.

Biomolecular condensate

Advertisement Hide. Preparation of surfactants by condensation of fatty acid esters with hydrolyzed proteins.

Authors Authors and affiliations O. Muscio B. Cole T.

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McCarty V. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Protein Hydrolysis

Google Scholar. Spivack, J. Linfield, Surfactant Science Series, Vol.